Application of serotyping and chromosomal restriction endonuclease digest analysis in investigating a laboratory-acquired case of Campylobacter jejuni enteritis.
نویسندگان
چکیده
A frequently passaged laboratory strain of Campylobacter jejuni was confirmed by serotyping on the basis of thermostable antigens and by bacterial chromosomal restriction endonuclease digests to be the causative agent of enteritis in a laboratory worker.
منابع مشابه
Ribosomal RNA gene restriction fragment diversity amongst Penner serotypes of Campylobacter jejuni and Campylobacter coli.
Diversity based on ribosomal RNA gene-restriction endonuclease digest patterns was detected amongst forty-seven strains of Campylobacter made up of 38 strains of Campylobacter jejuni and 9 strains of Campylobacter coli. Restriction digests of chromosomal DNA prepared by treating with Hae III were probed with an oligonucleotide specific for Campylobacter 16S ribosomal RNA genes. Seventeen distin...
متن کاملEvaluation of 10 methods to distinguish epidemic-associated Campylobacter strains.
We compared four phenotypic and six genotypic methods for distinguishing Campylobacter jejuni strains from animals and humans involved in four epidemics. Based on a comparison with epidemiologic data, the methods that correctly identified all strains in three milkborne outbreaks and one waterborne outbreak were heat-stable and heat-labile serotyping; multilocus enzyme electrophoresis (MEE); DNA...
متن کاملApplication of different chromosomal DNA restriction digest fingerprints to specific and subspecific identification of Campylobacter isolates.
Eleven strains comprising representatives of different subspecies, biotypes, and serotypes of Campylobacter jejuni and reference strains of C. coli, C. fetus subsp. fetus, C. hyointestinalis, and C. sputorum subsp. sputorum were studied to assess the utility of different DNA profiles for measuring fine differences between allied bacteria. Strains were compared by analyses of HaeIII and XhoI dig...
متن کاملبررسی میزان شیوع انتریت عفونی ناشی از کمپیلوباکترجوجنای در کودکان مبتلا به اسهال شهرستان خرم‌آباد
Background : Children's diarrhea is one of the major health problems worldwide, especially in developing countries, accounting for nearly 4-5 million deaths per year.Campylobacter jejuni is a common human bacterial pathogen, which plays at least the same leading role to Salmonella and Shigella as the causative agent of infectious diarrhea. Regarding limited studies on different epidemiologic as...
متن کاملSerotyping of Campylobacter jejuni/coli.
Antisera were prepared from strains of Campylobacter jejuni/coli isolated from patients in six outbreaks of enteritis. Bactericidal antibodies, and agglutinating antibodies to heat-labile and heat-stable antigens, were demonstrated. These reactions were used to type a number of strains isolated from patients in each outbreak, and to distinguish 'epidemic' from 'non-epidemic' strains.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 18 6 شماره
صفحات -
تاریخ انتشار 1983